Gaetti-Jardim E, Zelante F, Avila-Campos M J
Department of Microbiology, Instituto de Ciências Biomédicas, University of São Paulo, Brazil.
J Dent. 1996 Sep;24(5):345-8. doi: 10.1016/0300-5712(95)00084-4.
The aim of this study was the characterization and identification of oral Fusobacterium in patients with and without periodontal disease, and from spittoons and air-water syringes. The antimicrobial susceptibility of this bacterium was evaluated.
Subgingival samples were taken using sterilized absorbent paper points. Spittoon samples were collected using sterile swabs around the drain area with shut off, and air-water syringe samples by washing the tip with Ringer solution. Samples were transferred in tubes under CO2 flux. Diluted samples were inoculated on to Omata and Disraely agar and blood agar plates, which were incubated in anaerobiosis, at 37 degrees C, for 4 days. Bacterial species were identified biochemically. MIC was determined using an agar dilution method.
Periodontal patients, healthy subjects, spittoons and air-water syringes were 80%, 67.6%, 37.8% and 3.3% positive to Fusobacterium, respectively. Clindamycin, imipenem, lincomycin, metronidazole and tetracycline were active against all human and environmental isolates. Eighteen isolates resistant to ampicillin or penicillin G produced beta-lactamases. The presence of human oral bacteria in items of dental equipment supports the hypothesis that such equipment may serve as a vehicle for the transmission of pathogenic organisms.
Pieces of dental equipment may serve as a vehicle for the transmission of oral pathogenic organisms.
本研究旨在对患有和未患有牙周疾病的患者以及来自痰盂和空气 - 水注射器中的口腔梭杆菌进行特性鉴定和识别，并评估该细菌的抗菌药敏性。
使用无菌吸水纸尖采集龈下样本。使用无菌拭子在关闭排水区域周围采集痰盂样本，并用林格氏液冲洗空气 - 水注射器尖端采集样本。样本在二氧化碳气流下转移至试管中。将稀释后的样本接种于大玉和迪斯雷利琼脂以及血琼脂平板上，于37℃厌氧条件下孵育4天。通过生化方法鉴定细菌种类。采用琼脂稀释法测定最低抑菌浓度（MIC）。
牙周炎患者、健康受试者、痰盂和空气 - 水注射器中梭杆菌的阳性率分别为80%、67.6%、37.8%和3.3%。克林霉素、亚胺培南、林可霉素、甲硝唑和四环素对所有人类和环境分离株均有活性。18株对氨苄西林或青霉素G耐药的分离株产生β - 内酰胺酶。牙科设备物品中存在人类口腔细菌支持了这样一种假设，即此类设备可能是致病生物传播的载体。
牙科设备可能是口腔致病生物传播的载体。
