Preus H R, Lassen J, Aass A M, Christersson L A
Department of Periodontology, Dental Faculty, University of Oslo, Norway.
J Clin Periodontol. 1993 Apr;20(4):299-303. doi: 10.1111/j.1600-051x.1993.tb00362.x.
This study was designed to investigate whether antibiotic resistant micro-organisms are able to contaminate and survive on syringe tips used for subgingival deposition of antibiotics, and to test simple and effective means of disinfecting the syringe tip between applications. In the first part of the study, syringe tips used for application of Minocycline subgingival formula in 20 adult periodontitis patients were cultured for bacteria resistant to this drug before and after disinfecting them with ethanol. The results showed that 80% of the unwashed syringes were culture positive for minocycline resistant bacteria, whereas only 1 ethanol washed syringe tip was contaminated. In part II of the study, after dispensing minocycline periodontal formula in 20 patients, 10 of the syringe tips were washed with ethanol while 10 were left untreated. All syringes were stored in a refrigerator for 8 days, whereafter the tips were sampled for resistant bacteria. 20% of the unwashed tips were contaminated after 8 days incubation at 4 degrees C. None of the ethanol washed syringe tips were culture positive. We conclude that syringe tips may be contaminated with antibiotic resistant bacteria after dispensing the antibiotic in periodontal pockets. The transmission of these bacteria to other periodontal sites may be avoided by disinfecting the syringe tip with ethanol between applications. We have also shown that antibiotic resistant bacteria may survive on the syringe tip following 8 days storage in a refrigerator, suggesting that syringes used for subgingival deposition of an antibiotic should not be stored for reuse.
本研究旨在调查抗生素耐药微生物是否能够在用于龈下注射抗生素的注射器尖端污染并存活，并测试在两次注射之间对注射器尖端进行消毒的简单有效方法。在研究的第一部分，对20名成年牙周炎患者使用的用于注射米诺环素龈下配方的注射器尖端在用乙醇消毒前后进行了对该药物耐药细菌的培养。结果显示，80%未清洗的注射器对米诺环素耐药细菌培养呈阳性，而仅1个经乙醇清洗的注射器尖端被污染。在研究的第二部分，在给20名患者注射米诺环素牙周配方后，10个注射器尖端用乙醇清洗，10个未处理。所有注射器在冰箱中保存8天，之后对尖端取样检测耐药细菌。在4℃孵育8天后，20%未清洗的尖端被污染。经乙醇清洗的注射器尖端均未培养呈阳性。我们得出结论，在牙周袋注射抗生素后，注射器尖端可能被抗生素耐药细菌污染。通过在两次注射之间用乙醇对注射器尖端进行消毒，可以避免这些细菌传播到其他牙周部位。我们还表明，抗生素耐药细菌在冰箱中保存8天后可能在注射器尖端存活，这表明用于龈下注射抗生素的注射器不应储存以备再次使用。
