Deryckere F, McNair A, Gannon F
National Diagnostics Centre/Bio Research Ireland, Department of Microbiology, University College Galway, Ireland.
Gene. 1996 Oct 10;175(1-2):35-41. doi: 10.1016/0378-1119(96)00117-5.
We report the isolation and sequencing of a 1100-bp DNA fragment containing the salmon Hepatocyte Nuclear Factor 1 gene (sHNF1) promoter. The sHNF1 promoter cloned upstream of the chloramphenicol acetyl transferase (CAT) encoding gene is shown to be active in two cell lines of hepatic origin. DNasel footprint analysis of the proximal 400 bp reveals several protein-binding sites, including a CCAAT box, a potential site for Sp1, and three potential HNF4 binding sites. The sequence does not contain any canonical TATA box or initiator and sHNF1 transcription is initiated at four different sites spanning a region of 56 bp. Sequence comparison with the Xenopus laevis HNF1 promoter sequence did not show any significant similarity except in the region overlapping two of the potential HNF4 binding sites.
我们报告了一个包含鲑鱼肝细胞核因子1基因（sHNF1）启动子的1100碱基对DNA片段的分离和测序。克隆到氯霉素乙酰转移酶（CAT）编码基因上游的sHNF1启动子在两种肝源性细胞系中具有活性。对近端400碱基对进行的DNasel足迹分析揭示了几个蛋白质结合位点，包括一个CCAAT框、一个潜在的Sp1位点和三个潜在的HNF4结合位点。该序列不包含任何典型的TATA框或起始子，并且sHNF1转录在跨越56碱基对区域的四个不同位点起始。与非洲爪蟾HNF1启动子序列的序列比较显示，除了在与两个潜在HNF4结合位点重叠的区域外，没有任何显著的相似性。
