Kakikawa M, Oki M, Tadokoro H, Nakamura S, Taketo A, Kodaira K
Molecular Biology Group, Faculty of Engineering, Toyama University, Japan.
Gene. 1996 Oct 10;175(1-2):157-65. doi: 10.1016/0378-1119(96)00142-4.
Bacteriophage phi gle was induced from a lysogenic Lactobacillus strain Gle. phi gle genome is double-stranded DNA of approximately 42.5 kilo-base (kb) pairs. SDS poly-acrylamide gel electrophoresis demonstrated that the phage particles contain 4 major structural (capsid) proteins, gpB, gpG, gpO, and gpP, whose molecular weights (MW) are estimated to be 64, 43, 29 and 26 kilodaltons (kDa), respectively. More than 16 minor proteins ranging from 113 to 9.6 kDa were also detected. The genes for the major capsid proteins were cloned and each DNA sequence was determined. N-terminal amino acid alignments determined by protein sequencing completely coincided with those deduced from the nucleotide sequences.
噬菌体phi gle是从溶源性乳酸杆菌菌株Gle中诱导产生的。phi gle基因组是约42.5千碱基对（kb）的双链DNA。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表明，噬菌体颗粒含有4种主要结构（衣壳）蛋白，即gpB、gpG、gpO和gpP，其分子量（MW）估计分别为64、43、29和26千道尔顿（kDa）。还检测到16种以上分子量在113至9.6 kDa之间的次要蛋白。克隆了主要衣壳蛋白的基因并测定了每个DNA序列。通过蛋白质测序确定的N端氨基酸比对与从核苷酸序列推导的比对完全一致。
