霍乱弧菌C6706株中编码复制性DNA聚合酶III催化亚基的dnaE的克隆与特性分析
Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706.
作者信息
Franco A A, Yeh P E, Johnson J A, Barry E M, Guerra H, Maurer R, Morris J G
机构信息
Division of Infectious Diseases, University of Maryland School of Medicine, Baltimore 21201, USA.
出版信息
Gene. 1996 Oct 10;175(1-2):281-3. doi: 10.1016/0378-1119(96)00155-2.
We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the alpha (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.
我们报告称，霍乱弧菌（Vc）含有一个与大肠杆菌dnaE基因同源的基因，dnaE是复制性DNA聚合酶III（PolIII）的α（催化）亚基的结构基因。尽管编码的蛋白质中存在24%的氨基酸（aa）差异，但Vc基因能强烈互补大肠杆菌的dnaE温度敏感（ts）突变体，这表明复制所必需的所有功能特征都是保守的。
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