Iwagoe H, Kohrogi H, Fujii K, Hamamoto J, Hirata N, Ando M
Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Int Arch Allergy Immunol. 1996 Nov;111(3):291-8. doi: 10.1159/000237380.
We examined the effect of two different types of tyrosine kinase inhibitor, herbimycin A and genistein, on antigen-induced tracheal contraction and antigen-induced histamine release from the trachea in sensitized guinea pigs in vitro. Herbimycin A (1-10 microM) and genistein (1-10 microM) significantly inhibited antigen-induced tracheal contraction, compared with control. Additionally, herbimycin A (1-10 microM) and genistein (3-10 microM) significantly inhibited antigen-induced histamine release from the trachea in a concentration-dependent manner, compared with control. On the contrary, herbimycin A and genistein did not suppress acetylcholine- and histamine-induced tracheal contraction. We concluded that herbimycin A and genistein inhibit antigen-induced tracheal contraction without inhibiting smooth muscle contractility, and these inhibitory effects are due to, at least partially, inhibiting histamine release from tracheal mast cells.
我们在体外研究了两种不同类型的酪氨酸激酶抑制剂，即除莠霉素A和染料木黄酮，对致敏豚鼠抗原诱导的气管收缩以及气管中抗原诱导的组胺释放的影响。与对照组相比，除莠霉素A（1 - 10微摩尔）和染料木黄酮（1 - 10微摩尔）显著抑制了抗原诱导的气管收缩。此外，与对照组相比，除莠霉素A（1 - 10微摩尔）和染料木黄酮（3 - 10微摩尔）以浓度依赖的方式显著抑制了抗原诱导的气管组胺释放。相反，除莠霉素A和染料木黄酮并未抑制乙酰胆碱和组胺诱导的气管收缩。我们得出结论，除莠霉素A和染料木黄酮在不抑制平滑肌收缩性的情况下抑制抗原诱导的气管收缩，并且这些抑制作用至少部分归因于抑制气管肥大细胞释放组胺。
