Higashimoto Y, Ohata M, Nishio K, Iwamoto Y, Fujimoto H, Uetani K, Suruda T, Nakamura Y, Funasako M, Saijo N
Department of Internal Medicine, Wakayama Medical College Kihoku Hospital, Japan.
Anticancer Res. 1996 Sep-Oct;16(5A):2653-9.
The secosteroid 1 alpha, 25-dihydroxyvitamin D3 (calcitriol) and retinoic acid are the major biologically active metabolites of vitamins D and A, respectively. Their antitumor activity has been observed in several cancer cells in vitro apart from lung cancer cells. The purpose of this study was to examine the possible effects of the agents on lung cancer cell lines. The responses of five lung cancer cell lines to calcitriol or all-transretinoic acid (RA) were assessed by a colorimetric MTT assay. Calcitriol inhibited growth in one of the tested cell lines, i.e. EBC-1 squamous cell carcinoma, dose dependently. RA also exhibited the same effect in EBC-1 cells. However neither agent affected the growth of other lung cancer cell lines. Subsequently we examined the mRNA expression of vitamin D receptor (VDR) and retinoic acid receptor (RAR alpha) in these lung cancer cells by quantitative RT-PCR. EBC-1 cells expressed high levels of mRNA for both VDR and RAR alpha, while other cell lines expressed much lower mRNA levels for the receptors. These data suggest that the growth inhibitory effects of the vitamins are associated with mRNA expression for VDR and RAR alpha.
甾体类维生素D 1α,25 - 二羟基维生素D3（骨化三醇）和视黄酸分别是维生素D和维生素A的主要生物活性代谢产物。除肺癌细胞外，它们的抗肿瘤活性已在多种癌细胞体外实验中得到观察。本研究的目的是检测这些药物对肺癌细胞系的可能作用。采用比色法MTT实验评估了五种肺癌细胞系对骨化三醇或全反式维甲酸（RA）的反应。骨化三醇剂量依赖性地抑制了其中一种受试细胞系即EBC - 1鳞状细胞癌的生长。RA在EBC - 1细胞中也表现出相同的作用。然而，这两种药物均未影响其他肺癌细胞系的生长。随后，我们通过定量RT - PCR检测了这些肺癌细胞中维生素D受体（VDR）和视黄酸受体（RARα）的mRNA表达。EBC - 1细胞中VDR和RARα的mRNA表达水平较高，而其他细胞系中这两种受体的mRNA表达水平则低得多。这些数据表明，维生素的生长抑制作用与VDR和RARα的mRNA表达有关。
