Zerouga M, Stillwell W, Stone J, Powner A, Jenski L J
Department of Biology, Indiana University, Purdue University at Indianapolis 46202-5132, USA.
Anticancer Res. 1996 Sep-Oct;16(5A):2863-8.
Here we explore how incorporation of the omega-3 fatty acid docosahexaenoic acid (DHA) into murine leukemia cells (T27A) may alter membrane structure and function. When cells were cultured in DHA-supplemented medium, DHA incorporated rapidly and preferentially into phosphatidyl-ethanolamine (PE), with lesser and slower incorporation into phosphatidylcholine (PC). DHA at low concentrations preferred PE over neutral lipids, but in DHA excess accumulation in neutral lipids outstripped that of phospholipids. High DHA levels reduced cell growth in the apparent absence of lipid peroxidation. To study the importance of DHA's phospholipid class, cells were fused with lipid vesicles of either 18:0, 22:6 PE or 18:0, 22:6 PC. DHA-containing PC vesicles produced a dose-dependent decrease in cell viability, whereas PE-containing vesicles had little effect although they appeared more fusogenic. These results provoke the interesting speculation that T27A cells can safely accumulate DHA in PE, but are vulnerable if excessive DHA is incorporated into PC.
在此，我们探讨将ω-3脂肪酸二十二碳六烯酸（DHA）掺入鼠白血病细胞（T27A）中如何改变膜结构和功能。当细胞在添加DHA的培养基中培养时，DHA迅速且优先掺入磷脂酰乙醇胺（PE），而掺入磷脂酰胆碱（PC）的速度较慢且量较少。低浓度的DHA优先掺入PE而非中性脂质，但在DHA过量时，中性脂质中的积累超过了磷脂。在明显不存在脂质过氧化的情况下，高DHA水平会降低细胞生长。为了研究DHA在磷脂类别中的重要性，将细胞与18:0、22:6 PE或18:0、22:6 PC的脂质囊泡融合。含DHA的PC囊泡导致细胞活力呈剂量依赖性下降，而含PE的囊泡虽然似乎更具融合性，但影响很小。这些结果引发了一个有趣的推测，即T27A细胞可以在PE中安全地积累DHA，但如果过量的DHA掺入PC中则易受影响。
