Nakagawa T, Kubota T, Kabuto M, Kodera T
Department of Neurosurgery, Fukui Medical School, Japan.
Anticancer Res. 1996 Sep-Oct;16(5A):2917-22.
We investigated the effects of hyaluronic acid (HA) on the invasiveness of three human glioma cell lines (A172, T98G and U251) and their secretion of matrix metalloproteinases (MMPs), plasminogen activators (PAs), and hyaluronidase. The invasion of all three glioma cell lines was enhanced by the impregnation of Matrigel with HA in an in vitro invasion assay using 12 microns porosity polycarbonate filter transwells. The secretion of MMPs and PAs was not influenced by the presence of HA. Hyaluronidase activity was not detected in the culture media of any glioma cell line. HA also enhanced the motility of A172 and U251 glioma cells, but did not influence the motility of T98G glioma cells. The adhesion and spreading of all glioma cell lines were inhibited on HA-coated plates. HA, however, did not influence the proliferation of any of the glioma cell line. These results suggest that the presence of HA contributes to glioma cell invasion, which involves the stimulation of detachment and motility of glioma cells and the maintenance of proteinase secretion by glioma cells.
我们研究了透明质酸（HA）对三种人类胶质瘤细胞系（A172、T98G和U251）侵袭性及其基质金属蛋白酶（MMPs）、纤溶酶原激活剂（PAs）和透明质酸酶分泌的影响。在使用12微米孔隙率聚碳酸酯滤膜Transwell的体外侵袭试验中，用HA包被基质胶可增强所有三种胶质瘤细胞系的侵袭能力。HA的存在对MMPs和PAs的分泌没有影响。在任何胶质瘤细胞系的培养基中均未检测到透明质酸酶活性。HA还增强了A172和U251胶质瘤细胞的运动能力，但对T98G胶质瘤细胞的运动能力没有影响。在HA包被的平板上，所有胶质瘤细胞系的黏附和铺展均受到抑制。然而，HA对任何胶质瘤细胞系的增殖均无影响。这些结果表明，HA的存在有助于胶质瘤细胞的侵袭，这涉及刺激胶质瘤细胞的脱离和运动以及维持胶质瘤细胞的蛋白酶分泌。
