具有新特性的异源多聚体CLC氯离子通道。
Heteromultimeric CLC chloride channels with novel properties.
作者信息
Lorenz C, Pusch M, Jentsch T J
机构信息
Center for Molecular Neurobiology, Hamburg University, Germany.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13362-6. doi: 10.1073/pnas.93.23.13362.
Abstract
The skeletal muscle chloride channel CLC-1 and the ubiquitous volume-activated chloride channel CLC-2 belong to a large gene family whose members often show overlapping expression patterns. CLC-1 and CLC-2 are coexpressed in skeletal and smooth muscle and in the heart. By coexpressing CLC-1 and CLC-2 in Xenopus oocytes, we now show the formation of novel CLC-1/CLC-2 heterooligomers that yield time-independent linear chloride currents with a chloride-->bromide-->iodide selectivity sequence. Formation of heterooligomeric CLC channels increases the number and possible functions of chloride channels.
摘要
骨骼肌氯离子通道CLC-1和普遍存在的容积激活氯离子通道CLC-2属于一个大的基因家族，其成员的表达模式常常重叠。CLC-1和CLC-2在骨骼肌、平滑肌和心脏中共同表达。通过在非洲爪蟾卵母细胞中共表达CLC-1和CLC-2，我们现在展示了新型CLC-1/CLC-2异源寡聚体的形成，该异源寡聚体产生与时间无关的线性氯离子电流，具有氯离子>溴离子>碘离子的选择性序列。异源寡聚体CLC通道的形成增加了氯离子通道的数量和可能的功能。
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