Howard G C, DeCamp D L, Misra U K, Pizzo S V
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA.
Biochim Biophys Acta. 1996 Oct 17;1297(2):111-4. doi: 10.1016/s0167-4838(96)00135-5.
Site-directed mutagenesis of residues in the receptor binding fragment of rat alpha 1-macroglobulin identify residues 1377 and 1378 (human numbering) as crucial for interaction with the alpha 2M signaling receptor on murine macrophages. Secondary structure prediction and signaling studies with the mutant proteins suggest the alpha 2M signaling receptor binding site involves part of a loop-helix-loop motif comprised of residues 1372-1378.
对大鼠α1-巨球蛋白受体结合片段中的残基进行定点诱变，确定1377和1378位残基（人类编号）对于与小鼠巨噬细胞上的α2M信号受体相互作用至关重要。对突变蛋白的二级结构预测和信号研究表明，α2M信号受体结合位点涉及由1372 - 1378位残基组成的环-螺旋-环基序的一部分。
