Mouri T, Nakamura S, Ohyama Y, Matsuzaki G, Shinohara M, Kishihara K, Hiroki A, Oka M, Shirasuna K, Nomoto K
Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
Cancer Immunol Immunother. 1996 Sep;43(1):10-8. doi: 10.1007/s002620050297.
Oral squamous cell carcinomas (SCC) are often infiltrated by a large number of T lymphocytes. To clarify the nature of the tumour-infiltrating lymphocytes (TIL), we examined T cell receptor (TCR) V alpha and V beta gene usage by TIL and peripheral blood mononuclear cells (PBMC) obtained from 10 patients with oral SCC. We obtained RNA from TIL and PBMC, synthesized complementary DNA, and used the polymerase chain reaction (PCR) method with a panel of primers specific for the V gene segment subfamily (V alpha 1-18/V beta 1-20). We thus found that TIL showed more restricted usage of V beta gene families in contrast to PBMC of the same patients while two unique V beta gene (V beta 6 and V beta 5.2) segment transcripts were overexpressed in the TIL of more than half of the patients. On the other hand, no major difference was observed in the V alpha gene usage between the TIL and PBMC of most patients. To characterize these T cell subpopulations with unique V beta gene segment transcripts further, we sequenced the complementarity-determining region 3 in V beta 6-C beta and V beta 5.2-C beta PCR products derived from TIL and PBMC of two selected patients in each case. Although no usage of the conserved amino acid sequence by TIL was detected, the frequent use of V beta 6/J beta 1.1 in one patient and the V beta 6/J beta 2.7 gene segments in another patient was observed. Regarding the V beta 5.2 transcripts, obtained from the other two patients, no preferential usage of specific J beta gene segments by TIL was observed. These results suggest that the unique T cell populations are amplified in patients with oral SCC, possibly as a consequence of an in situ immune reaction.
口腔鳞状细胞癌（SCC）常被大量T淋巴细胞浸润。为阐明肿瘤浸润淋巴细胞（TIL）的性质，我们检测了10例口腔SCC患者的TIL和外周血单个核细胞（PBMC）的T细胞受体（TCR）Vα和Vβ基因使用情况。我们从TIL和PBMC中提取RNA，合成互补DNA，并使用针对V基因片段亚家族（Vα1 - 18/Vβ1 - 20）的一组引物通过聚合酶链反应（PCR）方法进行检测。结果发现，与同一患者的PBMC相比，TIL的Vβ基因家族使用更为受限，同时在超过半数患者的TIL中，两种独特的Vβ基因（Vβ6和Vβ5.2）片段转录本过度表达。另一方面，大多数患者的TIL和PBMC在Vα基因使用上未观察到明显差异。为进一步表征这些具有独特Vβ基因片段转录本的T细胞亚群，我们对每种情况下两名选定患者的TIL和PBMC衍生的Vβ6 - Cβ和Vβ5.2 - CβPCR产物中的互补决定区3进行了测序。虽然未检测到TIL使用保守氨基酸序列，但观察到一名患者频繁使用Vβ6/Jβ1.1，另一名患者频繁使用Vβ6/Jβ2.7基因片段。对于从另外两名患者获得的Vβ5.2转录本，未观察到TIL对特定Jβ基因片段的优先使用。这些结果表明，在口腔SCC患者中独特的T细胞群体被扩增，这可能是原位免疫反应的结果。
