Chiu H C, Chang C H, Chen J S, Jee S H
Department of Dermatology, National Taiwan University Hospital, Taipei, ROC.
J Formos Med Assoc. 1996 Sep;95(9):667-74.
The morphologic and functional characteristics of cultured hair follicle dermal papilla (DP) cells, dermal sheath (DS) cells and interstitial dermal fibroblasts (DF cells) derived from human scalp tissue are compared. DP and DS cells, but not DF cells, showed aggregative behavior at a preconfluent density. All three types of cells stained positive for type I collagen, type IV collagen, laminin and heparan sulfate proteoglycan. Only DP and DS cells expressed smooth muscle alpha-actin. DP and DS cells also synthesized more glycosaminoglycans (GAG) than DF cells, while there was no significant difference between DP and DS cells in GAG synthesis. Ultrastructurally, 7 out of 10 strains of DP and 2 out of 10 strains of DS cells were found to form intranuclear rodlets, while none of the 10 strains of DF cells examined formed intranuclear rodlets. The conditioned medium of the three types of cells was collected and tested for the presence of interleukin (IL)-1 beta, tumor necrosis factor (TGF)-beta 2, IL-6, platelet-derived growth factor-AB, epidermal growth factor, b-FGF, GM-CSF, insulin-like growth factor (IGF)-1 and HGF (hepatocyte growth factor) by ELISA or RIA. Among the tested cytokines and growth factors, TGF-beta 2, IL-6 and IGF-I were detectable in at least some conditioned media. The others were undetectable. There was no significant difference in the production of IL-6 and IGF-I among the three types of cells. In contrast, DP cells produced the highest levels of TGF-beta 2, DS cells produced intermediate levels of TGF-beta 2, and DF cells produced the lowest levels of TGF-beta 2. DP and DS cells are morphologically and functionally different from the nonfollicular, interstitial DF cells. Moreover, the presence of some minor biologic differences between DP and DS cells suggests that they represent follicular mesenchymal cells in different functional or differentiation states.
比较了源自人头皮组织的培养毛囊真皮乳头（DP）细胞、真皮鞘（DS）细胞和间质真皮成纤维细胞（DF细胞）的形态学和功能特征。DP和DS细胞，但不是DF细胞，在汇合前密度时表现出聚集行为。所有三种类型的细胞对I型胶原、IV型胶原、层粘连蛋白和硫酸乙酰肝素蛋白聚糖染色均呈阳性。只有DP和DS细胞表达平滑肌α-肌动蛋白。DP和DS细胞合成的糖胺聚糖（GAG）也比DF细胞多，而DP和DS细胞在GAG合成方面没有显著差异。在超微结构上，10株DP细胞中有7株和10株DS细胞中有2株形成核内小体，而所检测的10株DF细胞中均未形成核内小体。收集三种类型细胞的条件培养基，并通过ELISA或RIA检测白细胞介素（IL）-1β、肿瘤坏死因子（TGF）-β2、IL-6、血小板衍生生长因子-AB、表皮生长因子、b-FGF、GM-CSF、胰岛素样生长因子（IGF）-1和肝细胞生长因子（HGF）的存在。在所检测的细胞因子和生长因子中，至少在一些条件培养基中可检测到TGF-β2、IL-6和IGF-I。其他均未检测到。三种类型细胞在IL-6和IGF-I的产生上没有显著差异。相比之下，DP细胞产生的TGF-β2水平最高，DS细胞产生的TGF-β2水平中等，DF细胞产生的TGF-β2水平最低。DP和DS细胞在形态学和功能上与非毛囊间质DF细胞不同。此外，DP和DS细胞之间存在一些微小的生物学差异，表明它们代表处于不同功能或分化状态的毛囊间充质细胞。
