Ostiguy S, Gilbert M, Shareck F, Kluepfel D, Morosoli R
Centre de Recherche en Microbiologie Appliquée, Institut Armand-Frappier, Université du Québec, Canada.
Gene. 1996 Oct 17;176(1-2):265-7. doi: 10.1016/0378-1119(96)00229-6.
Two conserved regions of SecY proteins from six Gram+ bacteria were exploited in a PCR-based strategy for isolating a secY homolog from Streptomyces lividans (Sl). The nucleotide sequence of part of a 3.8-kb fragment showed that the secY homolog is flanked, at the 5' end, by the gene encoding ribosomal protein L15 and, at the 3' end, by an adenylate kinase-encoding gene. The deduced gene product of secY would have 437 amino acids (aa) and an M(r) of 47,200. Sl SecY shows 89.5, 56.1, 42 and 40% identity to its homologs from Streptomyces scabies, Brevibacterium flavum, Bacillus subtilis and Escherichia coli, respectively. Promoterprobe analyses indicated that the secY gene probably contains its own promoter.
