Feyereisen R
Experientia. 1977 Aug 15;33(8):1111-3. doi: 10.1007/BF01946001.
Specific binding of 3H-ecdysterone to a high mol. wt protein from Locusta migratoria haemolymph was shown by gel filtration. The hormone-protein complex shows a dissociation constant Kd congruent to 3.10(-7) M, and the concentration of binding sites varies during the last larval instar.