Shibata Y, Kotanagi H, Andoh H, Koyama K, Itoh H, Kudo S
Department of Surgery, Akita University School of Medicine, Japan.
Dis Colon Rectum. 1996 Nov;39(11):1269-74. doi: 10.1007/BF02055121.
Mutation of the p53 tumor suppressor gene is the most common genetic alternation in colorectal carcinoma and is assessed mainly by molecular analysis of the gene sequence or by immunohistochemical analysis of p53 protein accumulation. Purpose of this study was to detect circulating antibody against p53 proteins in serum of patients with colorectal carcinoma and to evaluate its clinical significance.
We used immunoblotting techniques to detect circulating anti-p53 antibodies. Relationship among staging, immunohistochemical expression of p53 in the primary tumor, and serum carcinoembryonic antigen level was investigated.
Circulating anti-p53 antibodies were detected in 32 (68 percent) of 47 patients. Antibody was found in patients at all clinical stages of disease. In 33 patients whose sera and primary tumor tissues were available for testing, 16 (48 percent) were positive for both circulating anti-p53 antibody and p53 expression in the tumor. The anti-p53 antibody test was positive in 78 and 56 percent of patients with high and normal serum carcinoembryonic antigen levels, respectively.
Detection of anti-p53 antibodies may become a new diagnostic indicator of colorectal carcinomas.
p53肿瘤抑制基因的突变是结直肠癌中最常见的基因改变，主要通过对基因序列的分子分析或对p53蛋白积累的免疫组织化学分析来评估。本研究的目的是检测结直肠癌患者血清中针对p53蛋白的循环抗体，并评估其临床意义。
我们使用免疫印迹技术检测循环抗p53抗体。研究了分期、原发肿瘤中p53的免疫组织化学表达与血清癌胚抗原水平之间的关系。
47例患者中有32例（68%）检测到循环抗p53抗体。在疾病的所有临床阶段的患者中均发现了抗体。在33例血清和原发肿瘤组织可用于检测的患者中，16例（48%）循环抗p53抗体和肿瘤中p53表达均为阳性。血清癌胚抗原水平高和正常的患者中，抗p53抗体检测阳性率分别为78%和56%。
抗p53抗体的检测可能成为结直肠癌的一种新的诊断指标。
