使用标准化的、内部对照的竞争性差异聚合酶链反应（CD-PCR）对慢性粒细胞白血病（CML）中的Bcr-Abl转录本进行定量分析。

Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR).

作者信息

Nagel S, Schmidt M, Thiede C, Huhn D, Neubauer A

机构信息

Abteilung für Innere Medizin m.S. Hämatologie und Onkologie, Virchow Klinikum, Medizinische Fakultät der Humboldt Universität zu Berlin, Germany.

出版信息

Nucleic Acids Res. 1996 Oct 15;24(20):4102-3. doi: 10.1093/nar/24.20.4102.

DOI:10.1093/nar/24.20.4102

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146207/

Abstract

The quantification of Bcr-Abl transcript numbers in chronic myelogenous leukemia (CML) patients described here uses simultaneous competitive PCR amplification of the target gene (Bcr-Abl) and a reference gene (porphobilinogen deaminase; Pbgd) together with a single composite competitor molecule for both targets based on heterologous sequences. Using this technique, Bcr-Abl transcript numbers could be reproducibly determined even in clinical samples known to harbour poor quality RNA.

摘要

本文所述的慢性髓性白血病（CML）患者中Bcr-Abl转录本数量的定量方法，是基于异源序列，使用针对靶基因（Bcr-Abl）和参考基因（胆色素原脱氨酶；Pbgd）的单一复合竞争分子，同时对这两个基因进行竞争性PCR扩增。使用该技术，即使在已知含有低质量RNA的临床样本中，也能可重复地测定Bcr-Abl转录本数量。

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本文引用的文献

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Competitive polymerase chain reaction to estimate the number of BCR-ABL transcripts in chronic myeloid leukemia patients after bone marrow transplantation.竞争性聚合酶链反应用于估计慢性髓性白血病患者骨髓移植后BCR-ABL转录本的数量。

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