Takashima K, Tateda K, Matsumoto T, Ito T, Iizawa Y, Nakao M, Yamaguchi K
Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
J Med Microbiol. 1996 Nov;45(5):319-22. doi: 10.1099/00222615-45-5-319.
Examination of strain differences in the susceptibility of mice to experimental respiratory tract infection with penicillin-resistant Streptococcus pneumoniae TUM19 revealed that a fatal infection model could be induced in immunocompetent CBA/J mice, but not in C3H/HeN, C57BL/6 or ICR mice. After intranasal instillation of c. 10(6) cfu of S. pneumoniae, the bacterial counts in the lungs of CBA/J mice increased from 10(5) to 10(7) cfu after 3-5 days, and gradually increased thereafter. The challenge organisms localised mainly in the lungs until 14 days after infection. Mice began to die c. 7 days after infection, and by 3 weeks most of the mice had died. Histopathologically, infiltration of neutrophils and lymphocytes around bronchi was observed from 1 day after infection, and fibrin deposition was seen in alveolar and bronchial spaces from 5 days. This model may be useful for investigating therapy of respiratory tract infection caused by penicillin-resistant S. pneumoniae because its pathological features resemble those observed in the human disease.
对小鼠感染青霉素耐药性肺炎链球菌TUM19所致实验性呼吸道感染易感性的品系差异研究表明，在免疫功能正常的CBA/J小鼠中可诱导出致死性感染模型，但在C3H/HeN、C57BL/6或ICR小鼠中则不能。经鼻内滴注约10⁶cfu的肺炎链球菌后，CBA/J小鼠肺部的细菌计数在3 - 5天后从10⁵cfu增加到10⁷cfu，此后逐渐增加。感染后14天内，攻击菌主要定位于肺部。小鼠在感染后约7天开始死亡，到3周时大多数小鼠已死亡。组织病理学检查显示，感染后1天即可观察到支气管周围有中性粒细胞和淋巴细胞浸润，5天后在肺泡和支气管腔内可见纤维蛋白沉积。该模型可能有助于研究由青霉素耐药性肺炎链球菌引起的呼吸道感染的治疗，因为其病理特征与人类疾病中观察到的相似。
