Valdivieso-Garcia A, MacLeod D L, Clarke R C, Gyles C L, Lingwood C, Boyd B, Durette A
Health of Animals Laboratory, Agriculture and Agri-Food Canada, Guelph, Ontario.
J Med Microbiol. 1996 Nov;45(5):331-7. doi: 10.1099/00222615-45-5-331.
Porcine and bovine aortic endothelial cells and human colonic adenocarcinoma cells were compared for their susceptibility to the toxic effect of purified Shiga-like toxin IIe (SLT-IIe), measured by the neutral red cytotoxicity assay. Cytotoxicity correlated with toxin binding as indicated by fluorescence activated cell sorter analysis and with the globotriosylceramide (Gb3) and globotetraosylceramide (Gb4) content of cells determined by high pressure liquid chromatography. One line of porcine aortic endothelial cells was 1400-fold more susceptible than the line of bovine aortic endothelial cells that was tested, but a second line of porcine aortic endothelial cells was highly refractory to SLT-IIe. Human colonic adenocarcinoma cells lacked detectable levels of Gb4 and were least susceptible to SLT-IIe.
通过中性红细胞毒性试验，比较猪和牛主动脉内皮细胞以及人结肠腺癌细胞对纯化的志贺样毒素IIe（SLT-IIe）毒性作用的敏感性。荧光激活细胞分选分析表明细胞毒性与毒素结合相关，高压液相色谱法测定的细胞中球三糖基神经酰胺（Gb3）和球四糖基神经酰胺（Gb4）含量也与细胞毒性相关。测试的一组猪主动脉内皮细胞比一组牛主动脉内皮细胞对SLT-IIe的敏感性高1400倍，但另一组猪主动脉内皮细胞对SLT-IIe具有高度抗性。人结肠腺癌细胞缺乏可检测水平的Gb4，对SLT-IIe最不敏感。
