Zerilli L F, Assandri A
Farmaco Sci. 1977 Aug;32(8):549-59.
Metabolic studies on diftalone (I), a new non-steroidal antiinflammatory agent, demonstated that 7-hydroxydiftalone beta-glucuronide (V) is the main urinary metabolite. The isolation of (V) from the urine of a dog treated with 14C-diftalone was performed by liquid-liquid partition after precolation through Amberlite XAD-2 and IRC-50 (H+). All the steps were followed by radio-detection and by thin layer radio-chromatography. Compound (V) was characterized mainly by mass spectrometry after esterification with diazomethane and silylation with BSA.
对新型非甾体抗炎药二氟拉松（I）的代谢研究表明，7-羟基二氟拉松β-葡糖醛酸苷（V）是主要的尿液代谢产物。在用14C-二氟拉松处理的犬尿液中分离（V）时，先通过Amberlite XAD-2和IRC-50（H+）进行预滤，然后进行液-液分配。所有步骤均通过放射性检测和薄层放射色谱法进行跟踪。化合物（V）主要通过用重氮甲烷酯化并用BSA进行硅烷化后的质谱进行表征。
