Blaser J, Triebel S, Maasjosthusmann U, Romisch J, Krahl-Mateblowski U, Freudenberg W, Fricke R, Tschesche H
Institut fur Biochemie der Universitat Bielefeld, Germany.
Clin Chim Acta. 1996 Jan 15;244(1):17-33. doi: 10.1016/0009-8981(95)06172-x.
The concentrations of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-8 (MMP-8), matrix metalloproteinase-9 (MMP-9), lactoferrin and urokinase plasminogen activator (uPA), tissue-type plasminogen activator (tPA) and the inhibitors, tissue inhibitor of metalloproteinase-1 (TIMP-1), plasminogen activator inhibitor-1 (PAI-1), plasminogen activator inhibitor (PAI-2), and alpha2-macroglobulin in the synovial fluids of patients with rheumatoid arthritis was determined before and during chemical synoviorthesis with a sodium salt of the fatty acids from cod-liver oil (Varicocid). Synovial fluids were obtained before treatment from 37 patients with rheumatoid arthritis and, in most cases, at 8 and 24 h after injection of the agent. Well-established ELISAs were used to determine the amounts of all proteins. All patients with rheumatoid arthritis revealed very high levels of metalloproteinases (about 1-15 mu g/ml) in their synovial fluids. During the inflammation inducing treatment the granulocyte enzymes increased. In contrast to this, the level of MMP-1 decreased. All granulocyte-derived enzymes were strongly correlated with each other, whereas their dependence on the granulocyte count was only weak. uPA and PAI-2 showed good correlations with the granulocytes-derived enzymes, but were also only weakly correlating with the cell counts. t-PA was not detected by the ELISA used. The proteases, MMP-8, MMP-9 and uPA were increased 8 h after the treatment, whereas the specific inhibitors TIMP-1, PAI-1 and PAI-2 showed significant changes only 24 h after the injection. Matrix metalloproteinases are important factors in the pathogenesis of rheumatoid arthritis. The inflammatory activity in the joint could be better correlated to the granulocyte enzymes than to the granulocyte counts. The levels of uPA and PAI-2 are also parallel to the granulocyte enzyme levels and might underly the same regulatory mechanism.
在使用鳕鱼肝油脂肪酸钠盐（Varicocid）进行化学滑膜切除术之前及过程中，测定类风湿性关节炎患者滑液中基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-8（MMP-8）、基质金属蛋白酶-9（MMP-9）、乳铁蛋白、尿激酶型纤溶酶原激活剂（uPA）、组织型纤溶酶原激活剂（tPA）以及抑制剂金属蛋白酶组织抑制剂-1（TIMP-1）、纤溶酶原激活剂抑制剂-1（PAI-1）、纤溶酶原激活剂抑制剂（PAI-2）和α2-巨球蛋白的浓度。在治疗前从37例类风湿性关节炎患者获取滑液，并且在大多数情况下，在注射该药剂后的8小时和24小时获取滑液。采用成熟的酶联免疫吸附测定法（ELISA）来测定所有蛋白质的含量。所有类风湿性关节炎患者的滑液中均显示出非常高水平的金属蛋白酶（约1 - 15μg/ml）。在诱导炎症的治疗过程中，粒细胞酶增加。与此相反，MMP-1的水平下降。所有源自粒细胞的酶彼此之间呈强相关，而它们与粒细胞计数的相关性较弱。uPA和PAI-2与源自粒细胞的酶显示出良好的相关性，但与细胞计数的相关性也较弱。所使用的ELISA未检测到t-PA。蛋白酶MMP-8、MMP-9和uPA在治疗后8小时增加，而特异性抑制剂TIMP-1、PAI-1和PAI-2仅在注射后24小时显示出显著变化。基质金属蛋白酶是类风湿性关节炎发病机制中的重要因素。关节中的炎症活动与粒细胞酶的相关性可能比与粒细胞计数的相关性更好。uPA和PAI-2的水平也与粒细胞酶水平平行，可能受相同的调节机制影响。
