一种使用聚合酶链反应检测番茄斑萎病毒属病毒的改进方法。

An improved method for the detection of Tospoviruses using the polymerase chain reaction.

作者信息

Mumford R A, Barker I, Wood K R

机构信息

School of Biological Sciences, University of Birmingham, UK.

出版信息

J Virol Methods. 1996 Mar;57(1):109-15. doi: 10.1016/0166-0934(95)01975-8.

DOI:10.1016/0166-0934(95)01975-8

PMID:8919828

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described.

摘要

一种基于逆转录聚合酶链反应（RT-PCR）的检测番茄斑萎病毒（TSWV）的分析方法已得到改进和扩展，以能够检测其他番茄斑萎病毒属病毒。除了TSWV特异性引物外，还设计了另外两对引物，一对专门检测凤仙花坏死斑病毒（INSV），另一对检测所有测试的番茄斑萎病毒属病毒，包括TSWV、INSV、番茄褪绿斑病毒和花生环斑病毒。还描述了一种改进的快速RNA提取方法。