鸡血浆中壮观霉素的简易酶联免疫吸附测定法。

Easy enzyme-linked immunosorbent assay for spectinomycin in chicken plasma.

作者信息

Tanaka T, Ikebuchi H, Sawada J I, Okada M, Kido Y

机构信息

Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, Kamiyoga, Tokyo, Japan.

出版信息

J AOAC Int. 1996 Mar-Apr;79(2):426-30.

PMID:8920128

Abstract

An easy, sensitive, competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) for spectinomycin in chicken plasma was developed. Preparation of a spectinomycin-bovine serum albumin conjugate in which the hapten is linked to the carrier protein through the C-4 position is described. Antibodies raised against antigens in rabbits had excellent specificity for spectinomycin, exhibiting a cross-reactivity of 44.0% with dihydrospectinomycin and 13.8% with tetrahydrospectinomycin. No cross-reactivity was observed with other antibiotics. The detection limit of the CI-ELISA was 2 ng/mL (equivalent into 40 ng/mL undiluted chicken plasma) spectinomycin. Known amounts (0.1-100 micrograms/mL) of spectinomycin were added to chicken plasma and then analyzed. Average recoveries were 97-110%. This procedure may be used without prior extraction of samples.

摘要

建立了一种简便、灵敏的鸡血浆中壮观霉素竞争间接酶联免疫吸附测定法（CI-ELISA）。描述了一种壮观霉素-牛血清白蛋白偶联物的制备方法，其中半抗原通过C-4位与载体蛋白相连。用兔制备的针对抗原的抗体对壮观霉素具有优异的特异性，与二氢壮观霉素的交叉反应率为44.0%，与四氢壮观霉素的交叉反应率为13.8%。未观察到与其他抗生素的交叉反应。CI-ELISA对壮观霉素的检测限为2 ng/mL（相当于未稀释鸡血浆中40 ng/mL）。将已知量（0.1-100微克/毫升）的壮观霉素加入鸡血浆中，然后进行分析。平均回收率为97-110%。该方法无需对样品进行预先提取即可使用。