液相色谱法测定苹果汁中展青霉素：协同研究

Determination of patulin in apple juice by liquid chromatography: collaborative study.

作者信息

Brause A R, Trucksess M W, Thomas F S, Page S W

机构信息

Analytical Chemical Services of Columbia, Inc., MD 21045, USA.

出版信息

J AOAC Int. 1996 Mar-Apr;79(2):451-5.

PMID:8920132

Abstract

An AOAC International-International Union of Pure and Applied Chemistry-International Fruit Juice Union (AOAC-IUPAC-IFJU) collaborative study was conducted to evaluate a liquid chromatographic (LC) procedure for determination of patulin in apple juice. Patulin is a mold metabolite found naturally in rotting apples. Patulin is extracted with ethyl acetate, treated with sodium carbonate solution, and determined by reversed-phase LC with UV detection at 254 or 276 nm. Water, water-tetrahydrofuran, or water-acetonitrile was used as mobile phase. Levels determined in spiked test samples were 20, 50, 100, and 200 micrograms/L. A test sample naturally contaminated at 31 micrograms/L was also included. Twenty-two collaborators in 10 countries analyzed 12 test samples of apple juice. Recoveries averaged 96%, with a range of 91-108%. Repeatability relative standard deviations (RSDr) ranged from 10.9 to 53.8%. The reproducibility relative standard deviation (RSDR) ranged from 15.1 to 68.8%. The LC method for determination of patulin in apple juice has been adopted first action by AOAC INTERNATIONAL.

摘要

开展了一项美国官方分析化学师协会（AOAC）国际组织-国际纯粹与应用化学联合会（IUPAC）-国际果汁联盟（IFJU）的合作研究，以评估一种用于测定苹果汁中展青霉素的液相色谱（LC）方法。展青霉素是一种在腐烂苹果中天然存在的霉菌代谢产物。展青霉素用乙酸乙酯萃取，用碳酸钠溶液处理，然后通过反相液相色谱在254或276nm处进行紫外检测来测定。水、水-四氢呋喃或水-乙腈用作流动相。加标测试样品中测定的水平为20、50、100和200微克/升。还包括一个天然污染水平为31微克/升的测试样品。来自10个国家的22名合作者分析了12个苹果汁测试样品。回收率平均为96%，范围为91%-108%。重复性相对标准偏差（RSDr）范围为10.9%至53.8%。再现性相对标准偏差（RSDR）范围为15.1%至68.8%。测定苹果汁中展青霉素的液相色谱方法已被AOAC国际组织首次采用。