苹果汁中展青霉素的快速反相液相色谱测定法。

Rapid reversed-phase liquid chromatographic determination of patulin in apple juice.

作者信息

Gökmen V, Acar J

机构信息

Department of Food Engineering, Hacettepe University, Beytepe, Ankara, Turkey.

出版信息

J Chromatogr A. 1996 Apr 12;730(1-2):53-8. doi: 10.1016/0021-9673(95)00861-6.

DOI:10.1016/0021-9673(95)00861-6

PMID:8680596

Abstract

A rapid, simple and economical method using a limited amount of organic solvent is described for the determination of patulin in apple juice. The sample was extracted with ethyl acetate and the extract was cleaned up by extraction with sodium carbonate solution. Patulin was then determined by reversed-phase liquid chromatography using a MicroPack C18 column and a variable-wavelength UV-Vis detector set at 276 nm. Patulin and 5-hydroxymethylfurfural were completely resolved by using water-acetonitrile (99:1, v/v) as the mobile phase at a flow-rate of 1.0 ml/min. The detection limit was < 5 micrograms/l and the recovery was 98%.

摘要

本文描述了一种快速、简单且经济的方法，该方法使用少量有机溶剂来测定苹果汁中的展青霉素。样品用乙酸乙酯萃取，萃取液用碳酸钠溶液萃取进行净化。然后使用MicroPack C18柱和设定在276nm的可变波长紫外可见检测器，通过反相液相色谱法测定展青霉素。以水 - 乙腈（99:1，v/v）为流动相，流速为1.0 ml/min时，展青霉素和5 - 羟甲基糠醛能完全分离。检测限小于5微克/升，回收率为98%。