Rubesa G, Beaman K D, Beer A E, Haller H, Rukavina D
Department of Physiology and Immunology, University of Rijeka, Croatia.
J Reprod Immunol. 1996 Feb;30(1):17-27. doi: 10.1016/0165-0378(96)00952-7.
TJ6, a newly described protein produced locally in the uterine decidua during pregnancy, may be involved in maintaining a unique immunological environment at the maternal-fetal interface. The aim of this study was to determine whether TJ6 is expressed as membrane form on decidual lymphocytes (DL), to define the phenotypes of TJ6m (membrane form TJ6) expressing cells and to analyze the fluorescence intensity of TJ6m expression. Peripheral blood lymphocytes (PBL) and DL were obtained from first trimester pregnancies undergoing elective termination and immunophenotyped for TJ6m and other cell surface antigens (CD3, CD8, CD19, CD56, CD16) by flow cytometry. This is the first study showing that TJ6 molecules are present on decidual lymphocytes in human pregnancy. TJ6m expression on PBL was not different from that of DL. However, a significantly higher percentage of double positive (TJ6m+CD3+, TJ6m+,CD8+,TJ6m+CD19+) cells were found in PBL when compared to DL. The average fluorescence intensity (AFI) for the TJ6m marker among cells with CD8+, CD19+ and CD56+ double positive was significantly higher in DL as compared with those of PBL. The AFI for granularity of double positive DL was significantly higher than observed in PBL.
TJ6是一种在孕期子宫蜕膜局部产生的新发现蛋白质，可能参与维持母胎界面独特的免疫环境。本研究旨在确定TJ6是否以膜形式在蜕膜淋巴细胞（DL）上表达，明确表达TJ6m（膜形式TJ6）的细胞表型，并分析TJ6m表达的荧光强度。从接受选择性终止妊娠的孕早期孕妇获取外周血淋巴细胞（PBL）和DL，通过流式细胞术对TJ6m和其他细胞表面抗原（CD3、CD8、CD19、CD56、CD16）进行免疫表型分析。这是第一项表明人类妊娠蜕膜淋巴细胞上存在TJ6分子的研究。PBL上的TJ6m表达与DL无异。然而，与DL相比，PBL中双阳性（TJ6m+CD3+、TJ6m+CD8+、TJ6m+CD19+）细胞的百分比显著更高。与PBL相比，DL中CD8+、CD19+和CD56+双阳性细胞中TJ6m标记的平均荧光强度（AFI）显著更高。双阳性DL的颗粒度AFI显著高于PBL。
