Kopreski M S, Witters L, Brennan W A, Buckwalter E A, Chinchilli V M, Demers L M, Lipton A
Clinical Research Branch, SAIC Frederick, National Cancer Institute, MD 21701, USA.
Anticancer Res. 1996 Sep-Oct;16(5B):3037-41.
Tyrosine kinase dependent oncogenes and growth factor receptors are of prognostic importance in breast cancer, but the relation of cytosolic protein tyrosine kinase (PTK) activity to traditional prognostic indicators is poorly defined. We determined cytosolic PTK activity in tumor extracts of 61 women with invasive breast cancer, including 51 primary specimens and 12 nodal or metastatic specimens, 7 women with in situ breast cancer, and 8 control breast specimens. PTK activity (pmol/min/mg) was measured in a dot blot assay using phosphotyrosine antibodies to detect phosphorylated tyrosyl residues in the tissue extracts. Compared with control specimens (mean PTK = 20.5), tyrosine kinase activity was significantly greater in invasive primary cancers (mean PTK = 298.1; p = 0.0008), and nodal/metastatic specimens (mean PTK = 491.5; p = 0.0009). PTK levels of invasive cancers did not correlate with age (p = 0.36), tumor size (p = 0.83), nodal status (p = 0.37), estrogen receptor status (p = 0.66), or progesterone receptor status (p = 0.09). Thus, while tyrosine kinase activity is increased in breast cancer, correlations with traditional prognostic indicators were not found.
酪氨酸激酶依赖性癌基因和生长因子受体在乳腺癌中具有预后意义，但胞质蛋白酪氨酸激酶（PTK）活性与传统预后指标的关系尚不明确。我们测定了61例浸润性乳腺癌女性患者肿瘤提取物中的胞质PTK活性，其中包括51例原发性标本和12例淋巴结或转移标本、7例原位乳腺癌女性患者以及8例对照乳腺标本。使用磷酸酪氨酸抗体通过斑点印迹法检测组织提取物中的磷酸化酪氨酰残基，从而测定PTK活性（pmol/分钟/毫克）。与对照标本（平均PTK = 20.5）相比，浸润性原发性癌症（平均PTK = 298.1；p = 0.0008）和淋巴结/转移标本（平均PTK = 491.5；p = 0.0009）中的酪氨酸激酶活性显著更高。浸润性癌症的PTK水平与年龄（p = 0.36）、肿瘤大小（p = 0.83）、淋巴结状态（p = 0.37）、雌激素受体状态（p = 0.66）或孕激素受体状态（p = 0.09）均无相关性。因此，虽然乳腺癌中酪氨酸激酶活性增加，但未发现其与传统预后指标存在相关性。
