Kuwashima Y, Kobayashi Y, Kawarai A, Kurosumi M, Tanuma J, Shiromizu K, Matsuzawa M, Kishi K
Department of Pathology, Saitama Cancer Center, Japan.
Anticancer Res. 1996 Sep-Oct;16(5B):3225-8.
In situ estimation of DNA fragmentation by the nick end labeling method and immunohistochemical detection of cell surface carbohydrate Le(y) were performed to establish correlation between the results of these two apoptosis-detecting techniques in human solid tumor tissues. Formalin-fixed and paraffin-embedded tissue samples from 10 cases of human endometrial adenocarcinoma were examined and compared by using the two techniques. A substantial fraction of carcinoma cells was found to be definitely stained by both methods, although Le(y) expression did not seem to be positively correlated with DNA fragmentation in the carcinoma tissue. These results suggest that Le(y) expression does not necessarily reflect apoptotic DNA fragmentation, at least in human endometrial adenocarcinomas, and simultaneous application of DNA nick end labeling and Le(y) immunostaining is necessary to show the relevant signs of apoptosis in histologic slides, especially gynecological cancers.
通过缺口末端标记法对DNA片段化进行原位评估，并对细胞表面碳水化合物Le(y)进行免疫组化检测，以确定这两种凋亡检测技术在人类实体瘤组织中的结果之间的相关性。使用这两种技术对10例人子宫内膜腺癌的福尔马林固定石蜡包埋组织样本进行了检查和比较。发现相当一部分癌细胞在两种方法中均被明确染色，尽管Le(y)表达在癌组织中似乎与DNA片段化无正相关。这些结果表明，至少在人子宫内膜腺癌中，Le(y)表达不一定反映凋亡性DNA片段化，并且同时应用DNA缺口末端标记和Le(y)免疫染色对于在组织学切片，尤其是妇科癌症中显示相关凋亡迹象是必要的。
