人体心脏组织的抗原分析——心脏抗原的特性鉴定
Antigenic analysis of human heart tissue--characterization of cardiac antigens.
作者信息
Kawakita S, Kato S, Nozawa T, Kinoshita M
出版信息
Jpn Circ J. 1979 May;43(5):452-7. doi: 10.1253/jcj.43.452.
The human heart antigens demonstrated in citric acid extracts by immunodiffusion were submitted to further analysis. Heart antigens were precipitated at 30% to 70% saturated ammonium sulphate. These fractions contained antigens reactive with rabbit antisera to human heart. The third fraction out of four proteins which were separated by Sephadex G-150 gel filtration, was reactive against rabbit antisera to human heart. The intensely stained proteins of both heart antigens were located at the nineth protein band out of proteins separated by polyacrylamide gel electrophoresis. This protein exhibited precipitin line against rabbit antisera to human heart by two-dimensional electrophoresis, and had mobility between that of serum alpha2 and beta1 globulin in immunoelectrophoresis.
通过免疫扩散在柠檬酸提取物中显示的人心脏抗原进行了进一步分析。心脏抗原在30%至70%饱和硫酸铵中沉淀。这些组分含有与兔抗人心脏抗血清反应的抗原。通过Sephadex G - 150凝胶过滤分离的四种蛋白质中的第三个组分，对兔抗人心脏抗血清有反应。通过聚丙烯酰胺凝胶电泳分离的蛋白质中，两种心脏抗原的强染色蛋白质位于第九个蛋白带。该蛋白质通过二维电泳显示出针对兔抗人心脏抗血清的沉淀线，并且在免疫电泳中的迁移率介于血清α2球蛋白和β1球蛋白之间。
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