Li Y, Yokoi T, Sasaki M, Hattori K, Katsuki M, Kamataki T
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Biochem Biophys Res Commun. 1996 Nov 12;228(2):312-7. doi: 10.1006/bbrc.1996.1658.
The expression and inducibility of CYP3A7 transgene in the fetus and suckling neonates from one of the transgenic lines (M10) were investigated by Northern and Western blot analyses. The mRNA expression could be detected as early as the 15th embryonic day and increased gradually with advancing gestation but then remarkably so after birth. The protein expression was also detectable postnatally and increased. Inducibility was achieved in neonatal mice via maternal exposure to zinc sulfate. Midazolam hydroxylase activities could be detected in liver microsomes prepared from 14-day-old neonates. These activities were significantly higher in transgenic than nontransgenic lines of mice (p < 0.001).
通过Northern印迹和Western印迹分析，研究了其中一个转基因品系（M10）的胎儿和哺乳期新生儿中CYP3A7转基因的表达及诱导性。早在胚胎第15天就能检测到mRNA表达，其随妊娠进展逐渐增加，但出生后显著增加。蛋白质表达在出生后也可检测到且有所增加。通过母体暴露于硫酸锌，在新生小鼠中实现了诱导性。在从14日龄新生儿制备的肝微粒体中可检测到咪达唑仑羟化酶活性。在转基因小鼠品系中，这些活性显著高于非转基因品系（p < 0.001）。
