VIII型胶原蛋白是发育和疾病过程中血管平滑肌细胞的产物。
Type VIII collagen is a product of vascular smooth-muscle cells in development and disease.
作者信息
MacBeath J R, Kielty C M, Shuttleworth C A
机构信息
Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, School of Biological Sciences, U.K.
出版信息
Biochem J. 1996 Nov 1;319 ( Pt 3)(Pt 3):993-8. doi: 10.1042/bj3190993.
Abstract
Type VIII collagen is a short-chain collagen with considerable similarity to type X collagen. We have generated chain-specific antibodies to the alpha 1 and alpha 2 chains of type VIII collagen, and used them as probes to examine the synthesis of type VIII collagen by vascular smooth muscle cells (VSMC). In addition, chain-specific oligonucleotides have been used in reverse transcriptase-PCR (RT-PCR) reactions with RNA extracted from cultured smooth muscle cells in culture and from freshly isolated vascular tissues. Radiolabelling of VSMC in culture and immunoprecipitation with chain-specific antibodies showed that both chains were expressed. Lower levels of type VIII collagen were found in adult VSMC than in neonatal VSMC. RT-PCR showed that both chains were expressed in tissues as well as cells in culture. The results indicate that type VIII collagen is a product of VSMC of normal adult vessels and is expressed at high levels by VSMC in vascular lesions.
摘要
VIII型胶原是一种与X型胶原具有相当相似性的短链胶原。我们制备了针对VIII型胶原α1和α2链的链特异性抗体，并将其用作探针来检测血管平滑肌细胞（VSMC）合成VIII型胶原的情况。此外，链特异性寡核苷酸已用于逆转录聚合酶链反应（RT-PCR），该反应使用从培养的平滑肌细胞以及新鲜分离的血管组织中提取的RNA。对培养的VSMC进行放射性标记并用链特异性抗体进行免疫沉淀表明两条链均有表达。在成年VSMC中发现的VIII型胶原水平低于新生VSMC。RT-PCR表明两条链在组织以及培养的细胞中均有表达。结果表明，VIII型胶原是正常成年血管VSMC的产物，并且在血管病变中的VSMC中高水平表达。
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本文引用的文献
1
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Am J Physiol. 1993 Jun;264(6 Pt 2):F1003-10. doi: 10.1152/ajprenal.1993.264.6.F1003.
2
Human mast cells produce type VIII collagen in vivo.人类肥大细胞在体内产生VIII型胶原蛋白。
Int J Exp Pathol. 1994 Dec;75(6):397-404.
3
Collagens: molecular biology, diseases, and potentials for therapy.胶原蛋白：分子生物学、疾病及治疗潜力
Annu Rev Biochem. 1995;64:403-34. doi: 10.1146/annurev.bi.64.070195.002155.
4
Type VIII collagen. Synthesis by normal and malignant cells in culture.VIII型胶原蛋白。培养的正常细胞和恶性细胞的合成。
Lab Invest. 1984 Feb;50(2):219-31.
5
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J Biol Chem. 1983 Nov 10;258(21):13391-401.
6
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J Biol Chem. 1983 Feb 25;258(4):2653-61.
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8
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9
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10
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