Smullen R P, David J A, Pitman R M
Institute of Aquaculture, University of Stirling, Scotland.
Gen Comp Endocrinol. 1996 Oct;104(1):84-91. doi: 10.1006/gcen.1996.0144.
The present study examines the mode of action of eyestalk factors, in particular crustacean hyperglycaemic hormone (CHH), on known target tissues (muscle and hepatopancreas) in two decapod crustacean species, Nephrops norvegicus and Carcinus maenas. The possibility that receptors to CHH are coupled to an elevation of cytosolic Ca2+ derived from intracellular stores via activation of the phosphatidylinositol pathway was investigated. Using an inositol 1,4,5-trisphosphate (InsP3)-specific competitive binding assay, measurable levels of InsP3 were detected in hepatopancreas and muscle in both N. norvegicus and C. maenas. Incubation of these tissues in the presence of sinus gland extracts (two sinus gland equivalents) consistently increased InsP3. In addition, Carcinus CHH (10 nM) increased InsP3 levels in Carcinus hepatopancreas and muscle. Incubation of hepatopancreas from both species in 10 mM lithium saline increased basal levels of InsP3, suggesting a significant turnover of phosphoinositides in this tissue.
本研究考察了眼柄因子，特别是甲壳类高血糖激素（CHH），对两种十足目甲壳类动物——挪威龙虾和绿青蟹已知靶组织（肌肉和肝胰腺）的作用方式。研究了CHH受体是否通过磷脂酰肌醇途径的激活与源自细胞内储存的胞质Ca2+升高相偶联的可能性。使用肌醇1,4,5-三磷酸（InsP3）特异性竞争性结合测定法，在挪威龙虾和绿青蟹的肝胰腺和肌肉中均检测到了可测量水平的InsP3。在窦腺提取物（两个窦腺当量）存在的情况下孵育这些组织，InsP3持续增加。此外，绿青蟹CHH（10 nM）增加了绿青蟹肝胰腺和肌肉中的InsP3水平。将两种物种的肝胰腺在10 mM锂盐溶液中孵育会增加InsP3的基础水平，表明该组织中磷酸肌醇有显著的周转。
