Aman P, Panagopoulos I, Lassen C, Fioretos T, Mencinger M, Toresson H, Höglund M, Forster A, Rabbitts T H, Ron D, Mandahl N, Mitelman F
Department of Clinical Genetics, University Hospital of Lund, Sweden.
Genomics. 1996 Oct 1;37(1):1-8. doi: 10.1006/geno.1996.0513.
FUS (TLS) was first identified as the 5'-part of a fusion gene with CHOP (GADD153, DDIT3) in myxoid liposarcomas with t(12; 16)(q13; p11). Homologies were found with the EWS oncogene, which is rearranged in Ewing sarcomas and other neoplasias. The genomic structure of FUS shows extensive similarities with that of EWS, but the exon/intron structures differ in the 5' parts, and overall FUS is smaller than EWS. Exon 3 of FUS corresponds to exons 3 and 4 in EWS. FUS exons 4-6 correspond to EWS exons 5-8. Exons 7 to 15 of FUS are very similar to those in EWS, although the EWS exons are larger than the corresponding FUS exons. FUS and EWS were expressed in all tissues investigated. The transcripts were stable within the 160-min half-life experiments. No or little variation in FUS or EWS expression was seen when resting lymphocytes were activated. These observations indicate that FUS and EWS belong to the housekeeping type of genes. This view is supported by the presence of the housekeeping gene type of promoter region in both genes.
FUS（TLS）最初是在伴有t（12；16）（q13；p11）的黏液样脂肪肉瘤中作为与CHOP（GADD153，DDIT3）融合基因的5'部分被鉴定出来的。发现它与EWS癌基因存在同源性，EWS在尤因肉瘤和其他肿瘤中发生重排。FUS的基因组结构与EWS的基因组结构有广泛的相似性，但外显子/内含子结构在5'部分有所不同，并且总体上FUS比EWS小。FUS的外显子3对应于EWS的外显子3和4。FUS的外显子4 - 6对应于EWS的外显子5 - 8。FUS的外显子7至15与EWS中的外显子非常相似，尽管EWS的外显子比相应的FUS外显子大。FUS和EWS在所研究的所有组织中均有表达。在160分钟半衰期实验中，转录本是稳定的。当静息淋巴细胞被激活时，未观察到FUS或EWS表达有变化或仅有轻微变化。这些观察结果表明FUS和EWS属于管家基因类型。这一观点得到了两个基因中管家基因类型启动子区域存在的支持。
