Collin G B, Münch A, Mu J L, Naggert J K, Olsen A S, Nishina P M
Jackson Laboratory, Bar Harbor, Maine 04609-1500, USA.
Genomics. 1996 Oct 1;37(1):125-30. doi: 10.1006/geno.1996.0529.
We describe here the identification of 11 novel microsatellite polymorphisms on human chromosome 19. These dinucleotide repeat polymorphisms were detected in chromosome 19-specific cosmids that were physically mapped by fluorescence in situ hybridization. For each repeat, flanking oligonucleotide primers were synthesized and the polymerase chain reaction assay was performed on a panel of 100 unrelated individuals to determine the heterozygosity and allele frequencies. To characterize these markers further, genetic and radiation hybrid maps were constructed. These microsatellite polymorphisms will be valuable in further linkage analysis of inherited diseases on chromosome 19p.
我们在此描述了在人类19号染色体上鉴定出的11种新的微卫星多态性。这些二核苷酸重复多态性是在通过荧光原位杂交进行物理定位的19号染色体特异性黏粒中检测到的。对于每个重复序列，合成了侧翼寡核苷酸引物，并在一组100个无关个体上进行聚合酶链反应分析，以确定杂合性和等位基因频率。为了进一步表征这些标记，构建了遗传图谱和辐射杂种图谱。这些微卫星多态性对于19号染色体短臂上遗传性疾病的进一步连锁分析将具有重要价值。
