在酵母双杂交系统中UBE2I与RAD52、UBL1、p53和RAD51蛋白的关联。

Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system.

作者信息

Shen Z, Pardington-Purtymun P E, Comeaux J C, Moyzis R K, Chen D J

机构信息

Life Sciences Division, Los Alamos National Laboratories, New Mexico 87545, USA.

出版信息

Genomics. 1996 Oct 15;37(2):183-6. doi: 10.1006/geno.1996.0540.

DOI:10.1006/geno.1996.0540

PMID:8921390

Abstract

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.

摘要

酵母中依赖RAD52的途径参与DNA重组和双链断裂修复。酵母泛素结合酶UBC9参与S期和M期细胞周期蛋白的降解及有丝分裂调控。在酵母双杂交系统中，以人RAD52蛋白作为“诱饵”，我们鉴定出了酵母UBC9的一个人类同源物，命名为UBE2I，它可与RAD52、RAD51、p53及一种类泛素蛋白UBL1相互作用。这些相互作用具有UBE2I特异性，因为另一种与DNA修复相关的泛素结合酶RAD6（UBC2）不与这些蛋白相互作用。UBE2I与RAD52的相互作用由RAD52的自缔合区域介导。这些结果表明，依赖RAD52的过程、细胞周期调控、p53介导的途径以及泛素化作用通过人UBE2I相互作用。