Taguchi F, Okuda S, Uchino U, Muraoka H, Hasegawa M, Kobayashi I
Department of Microbiology, Kitasato University School of Allied Health Sciences.
Kansenshogaku Zasshi. 1996 Sep;70(9):938-46. doi: 10.11150/kansenshogakuzasshi1970.70.938.
PMAC agar, a novel, selective and differential medium has been developed and was subjected for evaluation of its selective and differential capability of methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa from other bacteria such as Bacillus, Micrococcus, Gram-negative bacteria and drug resistant ones. Growth of MRSA and P. aeruginosa on PMAC agar was facilitated and their colonies were easily differentiated. Colonies of MRSA after 24 approximately 48 h incubation at 35 degrees C were small (2 to 4 mm in diameter), smooth and egg-yolk reaction positive. On the other hand, P. aeruginosa with pigment production (pyocianin, fluorescin or pyomelanin) formed large (2.5 to 7.0 mm in diameter), brownish black or brown colonies with a creamy edge. PMAC agar did not allow to grow unwanted bacteria tested except certain species formerly classified to Pseudomonas such as Burkholderia and Stenotrophomonas. However multi-drug resistant strains such as Enterobacter cloacae, Serratia marcescens and Acinetobacter calcoaceticus formed extremely small colonies. PMAC agar is recommended as a novel, useful medium for isolation, differentiation and presumptive identification of MRSA and P. aeruginosa from clinical and environmental sources.
PMAC琼脂是一种新型的选择性鉴别培养基，已被开发出来，并用于评估其从芽孢杆菌、微球菌、革兰氏阴性菌和耐药菌等其他细菌中选择性分离并鉴别耐甲氧西林金黄色葡萄球菌（MRSA）和铜绿假单胞菌的能力。MRSA和铜绿假单胞菌在PMAC琼脂上易于生长，其菌落也易于区分。MRSA在35℃培养约24至48小时后的菌落较小（直径2至4毫米），表面光滑，蛋黄反应呈阳性。另一方面，产生色素（绿脓菌素、荧光素或脓黑素）的铜绿假单胞菌形成大的（直径2.5至7.0毫米）、棕黑色或棕色菌落，边缘呈奶油色。除了某些以前归类为假单胞菌属的菌种（如伯克霍尔德菌属和嗜麦芽窄食单胞菌）外，PMAC琼脂不允许测试的其他有害细菌生长。然而，阴沟肠杆菌、粘质沙雷氏菌和醋酸钙不动杆菌等多重耐药菌株形成的菌落极小。PMAC琼脂被推荐为一种新型的、有用的培养基，用于从临床和环境样本中分离、鉴别和初步鉴定MRSA和铜绿假单胞菌。
