Fuchino Y, Okada N, Funabashi K, Otsuka K, Tomita A, Shinohara T, Ikuno T, Okada H
First Department of Surgery, School of Medicine, Fukuoka University.
Nihon Ronen Igakkai Zasshi. 1996 Aug;33(8):580-5. doi: 10.3143/geriatrics.33.580.
Decay-accelerating factor (DAF) is a membrane glycoprotein that prevents complement activation on blood cells. Among CD8+ T cells, DAF-negative cells can be distinguished from DAF-positive cells. We computed the proportion of DAF-negative CD8+ T cells in the peripheral blood of 59 normal healthy subjects, 27 to 93 years old, and analyzed the differences between subjects of different ages. The proportion of CD8+ T cells that were DAF-negative correlated significantly and positively with age. We also studied these lymphocytes in patients with cerebrovascular dementia, Alzheimer's dementia, cancer, rheumatoid arthritis and systemic lupus erythematosus. The proportion of CD8+ T cells that were DAF-negative did not correlate significantly with age in patients with cerebrovascular dementia, Alzheimer's dementia or cancer, but it correlated significantly and positively with age in patients with rheumatoid arthritis and in those with systemic lupus erythematosus. Therefore, healthy subjects and patients with various diseases can be classified according to age and to the proportion of CD8+ T cells that are DAF-negative. This proportion can then be used as an index of aging and of host defense function.
衰变加速因子（DAF）是一种膜糖蛋白，可防止补体在血细胞上激活。在CD8 + T细胞中，DAF阴性细胞可与DAF阳性细胞区分开来。我们计算了59名年龄在27至93岁之间的正常健康受试者外周血中DAF阴性CD8 + T细胞的比例，并分析了不同年龄受试者之间的差异。DAF阴性的CD8 + T细胞比例与年龄呈显著正相关。我们还研究了脑血管性痴呆、阿尔茨海默病性痴呆、癌症、类风湿性关节炎和系统性红斑狼疮患者的这些淋巴细胞。在脑血管性痴呆、阿尔茨海默病性痴呆或癌症患者中，DAF阴性的CD8 + T细胞比例与年龄无显著相关性，但在类风湿性关节炎患者和系统性红斑狼疮患者中，该比例与年龄呈显著正相关。因此，健康受试者和患有各种疾病的患者可根据年龄以及DAF阴性的CD8 + T细胞比例进行分类。然后，该比例可作为衰老和宿主防御功能的指标。
