Doerig C M, Parzy D, Langsley G, Horrocks P, Carter R, Doerig C D
Unité INSERM 399, Faculté de Médecine de Marseille, France.
Gene. 1996 Oct 24;177(1-2):1-6. doi: 10.1016/0378-1119(96)00281-8.
Pfmap-1, a gene encoding a novel protein kinase, has been identified in the human malaria parasite Plasmodium falciparum, using the polymerase chain reaction with degenerate oligodeoxyribonucleotides designed to hybridise to conserved regions of cdc2-related kinases. Computer comparison with other protein kinases strongly suggests that the protein encoded by this gene is closely related to mitogen-activated protein (MAP) kinases, which play important roles in eukaryotic adaptative response and signal transduction. In addition to the conserved MAP kinase catalytic domain, Pfmap-1 contains a highly charged C-terminal extension that includes two sets of repeated amino acid motifs. Pfmap-1 is located on chromosome 14 of P.falciparum, and its mRNA has a size of 3.7 kb.
Pfmap-1是一种编码新型蛋白激酶的基因，它是在人类疟原虫恶性疟原虫中通过聚合酶链反应与设计用于与细胞周期蛋白依赖性激酶2（cdc2）相关激酶保守区域杂交的简并寡脱氧核糖核苷酸鉴定出来的。与其他蛋白激酶的计算机比较强烈表明，该基因编码的蛋白质与丝裂原活化蛋白（MAP）激酶密切相关，后者在真核生物适应性反应和信号转导中起重要作用。除了保守的MAP激酶催化结构域之外，Pfmap-1还包含一个高度带电的C末端延伸，其中包括两组重复的氨基酸基序。Pfmap-1位于恶性疟原虫的14号染色体上，其mRNA大小为3.7 kb。
