White J H, Kilbey B J, de Vries E, Goman M, Alano P, Cheesman S, McAleese S, Ridley R G
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Nucleic Acids Res. 1993 Aug 11;21(16):3643-6. doi: 10.1093/nar/21.16.3643.
The gene encoding DNA polymerase alpha from the human malaria parasite Plasmodium falciparum has been sequenced and characterised. The deduced amino acid sequence possesses the seven sequence motifs which characterise eukaryotic replicative DNA polymerases (I-VII) and four of five motifs (A-E) identified in alpha DNA polymerases. The predicted protein also contains sequences which are reminiscent of Plasmodium proteins but absent from other DNA polymerases. These include four blocks of additional amino acids interspersed with the conserved motifs of the DNA polymerases, four asparagine rich sequences and a novel carboxy-terminal extension. Repetitive sequences similar to those found in other malarial proteins are also present. cDNA-directed PCR was used to establish the presence of these features in the approximately 7kb mRNA. The coding sequence contains a single intron. The gene for DNAPol alpha is located on chromosome 4 and is transcribed in both asexual and sexual erythrocytic stages of the parasite.
已对人类疟原虫恶性疟原虫编码DNA聚合酶α的基因进行了测序和表征。推导的氨基酸序列具有表征真核生物复制性DNA聚合酶的七个序列基序（I - VII）以及在α DNA聚合酶中鉴定出的五个基序（A - E）中的四个。预测的蛋白质还包含一些序列，这些序列让人联想到疟原虫蛋白质，但在其他DNA聚合酶中不存在。这些包括穿插在DNA聚合酶保守基序中的四个额外氨基酸块、四个富含天冬酰胺的序列和一个新的羧基末端延伸。还存在与其他疟疾蛋白质中发现的类似的重复序列。利用cDNA定向PCR确定了这些特征在约7kb mRNA中的存在。编码序列包含一个内含子。DNAPolα基因位于4号染色体上，在寄生虫的无性和有性红细胞阶段均有转录。
