Krausz E, Graw J
Institut für Säugetiergenetik, GSF-Forschungszentrum für Umwelt und Gesundheit, Neuherberg, Oberschleissheim, Germany.
Gene. 1996 Oct 24;177(1-2):99-102. doi: 10.1016/0378-1119(96)00280-6.
A cat reporter gene plasmid was constructed, which can be used very efficiently to clone PCR-derived promotor and enhancer fragments from genomic DNA. The new vector system pEK0CAT combines the efficiency in cloning with the approved low background of the pBLCAT6 vector. Additionally, the plasmid pEKSVCAT was constructed including the SV40 early promoter/enhancer to efficiently drive the cat reporter gene in particular cell lines. It can be used to optimize transfection conditions and as an internal positive control.
构建了一种猫报告基因质粒，它可非常有效地用于从基因组DNA中克隆PCR衍生的启动子和增强子片段。新的载体系统pEK0CAT将克隆效率与已获认可的低背景pBLCAT6载体相结合。此外，构建了质粒pEKSVCAT，其包含SV40早期启动子/增强子，以在特定细胞系中有效驱动猫报告基因。它可用于优化转染条件并作为内部阳性对照。
