Fedorova N D, Peredelchuk M Y, Kirpichnikov M P, Bennett G N
Engelhardt Institute of Molecular Biology, Moscow, Russia.
Gene. 1996 Oct 24;177(1-2):267-8. doi: 10.1016/0378-1119(96)00294-6.
An Escherichia coli strain for thermoinducible T7Pol-driven transcription has been constructed. The strain was obtained by site-specific integration of the T7 gene 1 coding for T7Pol into the attB site of phage lambda in the E. coli chromosome. The expression of the inserted gene is regulated by cIts857 and major early promoter-operator regions of phage lambda.
构建了一种用于热诱导T7聚合酶驱动转录的大肠杆菌菌株。该菌株通过将编码T7聚合酶的T7基因1位点特异性整合到大肠杆菌染色体中噬菌体λ的attB位点而获得。插入基因的表达受cIts857和噬菌体λ的主要早期启动子 - 操纵子区域调控。
