Zagdańska B, Wiśniewski K
Plant Physiology and Biochemistry Department, Plant Breeding and Acclimation Institute, Warsaw, Poland.
Acta Biochim Pol. 1996;43(3):515-9.
In wheat leaves acclimated and non-acclimated to water deficit the azocaseinolytic activities of endoproteinases were increased about 7-fold under drought conditions. Under such conditions both the pH dependence profile and the endoproteinase pattern were also changed. The predominant contribution of serine proteinase (about 50% of total endoproteinase activity) remains unaltered in the drought stressed leaves. Cysteine proteinase was induced to the same extent in the drought-stressed leaves irrespective of the acclimation pretreatment, while the contribution of aspartic proteinase was reduced upon water deficit but in the acclimated stressed leaves was as high as in the non-stressed leaves. These changes in the pattern of endoproteinases seem to imply that the water deficiency affects endogenous proteolysis.
在适应和未适应水分亏缺的小麦叶片中，干旱条件下内蛋白酶的酪蛋白分解活性增加了约7倍。在这种条件下，pH依赖性图谱和内蛋白酶模式也发生了变化。丝氨酸蛋白酶的主要贡献（约占总内蛋白酶活性的50%）在干旱胁迫叶片中保持不变。无论适应预处理如何，干旱胁迫叶片中半胱氨酸蛋白酶的诱导程度相同，而天冬氨酸蛋白酶的贡献在水分亏缺时降低，但在适应胁迫叶片中与非胁迫叶片中一样高。内蛋白酶模式的这些变化似乎意味着水分亏缺会影响内源性蛋白水解。
