Monnerie H, Boespflug-Tanguy O, Dastugue B, Meiniel A
INSERM U 384, Faculté de Médecine, Clermont-Ferrand, France.
Brain Res Dev Brain Res. 1996 Oct 23;96(1-2):120-9. doi: 10.1016/0165-3806(96)00106-x.
The subcommissural organ (SCO), which belongs to the circumventricular organs, is a specialized ependymal structure of the brain that secretes glycoproteins into the cerebrospinal fluid (CSF) which condense to form a thread-like structure, Reissner's fiber (RF). The effects of soluble material released by RF were examined in primary cultures of dissociated cortical cells from embryonic (day 8) chick brain. Under serum-free conditions, the presence in the cultures of soluble RF material markedly impaired neuronal cell aggregation. This effect was completely blocked by addition into the culture medium of specific antibodies raised against bovine RF. The anti-aggregative effect of soluble RF material is observed on poly-L-lysine as well as on different extracellular matrix proteins including collagen and laminin, but was less effective on fibronectin. The continuous exposure of the cultures to soluble RF material for 7 days significantly decreased choline acetyltransferase activity. On the other hand, soluble RF material did not appear to have mitogenic activity on neuronal cultures. Modulation of cell-cell interactions by SCO/RF glycoproteins strengthens the hypothesis of the involvement of RF in developmental events of the central nervous system.
连合下器官（SCO）属于室周器官，是大脑中的一种特殊室管膜结构，它将糖蛋白分泌到脑脊液（CSF）中，这些糖蛋白凝聚形成一种丝状结构，即赖斯纳纤维（RF）。在来自胚胎（第8天）鸡脑的解离皮质细胞的原代培养物中检测了RF释放的可溶性物质的作用。在无血清条件下，培养物中可溶性RF物质的存在显著损害神经元细胞聚集。向培养基中添加针对牛RF产生的特异性抗体可完全阻断这种作用。可溶性RF物质的抗聚集作用在聚-L-赖氨酸以及包括胶原蛋白和层粘连蛋白在内的不同细胞外基质蛋白上均有观察到，但对纤连蛋白的作用较弱。将培养物连续暴露于可溶性RF物质7天可显著降低胆碱乙酰转移酶活性。另一方面，可溶性RF物质似乎对神经元培养物没有促有丝分裂活性。SCO/RF糖蛋白对细胞间相互作用的调节强化了RF参与中枢神经系统发育事件的假说。
