Corrado M U, Pelli P, Chessa M G, Margallo E, Lattes A, Nicolò G
Institute of Zoology, University of Genoa, Italy.
Eur J Histochem. 1996;40(3):181-8.
Resting encystment is a reversible cytodifferentiation phenomenon regularly taking place during the life cycle of Colpodidae. The encystment involves regulation of the macronuclear DNA content, accomplished via chromatin extrusion and DNA synthesis mechanisms. An analysis of macronuclear chromatin texture was carried out in Colpoda inflata logarithmically growing cells, pre-cystic cells, and increasingly aging resting cysts after Feulgen staining. Morphometric and densitometric parameters as well as Markovian chromatin texture variables were utilised for image analysis. Moreover, univariate and multivariate analyses were applied both to macronuclei and to their chromatin extrusion bodies. The results show that structural variations in chromatin appear under macronuclear functional activity conditions, suggesting a dynamic state of chromatin texture throughout a long-term resting encystment.
静止包囊化是一种在肾形虫科生命周期中定期发生的可逆细胞分化现象。包囊化涉及通过染色质挤出和DNA合成机制对大核DNA含量的调节。在用福尔根染色后，对对数生长期的膨胀肾形虫细胞、囊前期细胞以及逐渐老化的静止包囊进行了大核染色质结构分析。形态测量和密度测量参数以及马尔可夫染色质结构变量被用于图像分析。此外，单变量和多变量分析既应用于大核，也应用于它们的染色质挤出体。结果表明，染色质的结构变化出现在大核功能活性条件下，这表明在长期静止包囊化过程中染色质结构处于动态状态。
