Goto K, Kimura T, Goto M, Sato H
Gan. 1977 Apr;68(2):227-31.
In the medium containing dextran sulfate, 3T6 cells stopped growth at almost monolayer stage and showed decreased saturation density. This action of dextran sulfate is referred to as a growth restriction. About 70% of untreated 3T6 cells formed piled-up colonies whereas the remaining 30% formed monolayer colonies. Fraction of the latter increased with increasing concentration of dextran sulfate in the medium. Cell lines sensitive and insensitive to the growth-restricting action of the compound were cloned from colonical culture of the wild type cells. Sensitive cells showed a slightly less saturation density than the wild type cells in the presence of dextran sulfate. Insensitive cells grown in dextran sulfate medium showed almost the same growth rate and saturation density as the untreated wild type cells grown in the absence of the compound.
在含有硫酸葡聚糖的培养基中，3T6细胞在接近单层阶段时停止生长，并表现出饱和密度降低。硫酸葡聚糖的这种作用被称为生长限制。约70%未处理的3T6细胞形成堆积菌落，而其余30%形成单层菌落。后者的比例随着培养基中硫酸葡聚糖浓度的增加而增加。从野生型细胞的克隆培养物中克隆出对该化合物生长限制作用敏感和不敏感的细胞系。在存在硫酸葡聚糖的情况下，敏感细胞的饱和密度略低于野生型细胞。在硫酸葡聚糖培养基中生长的不敏感细胞与在无该化合物情况下生长的未处理野生型细胞表现出几乎相同的生长速率和饱和密度。
