De clercq L, Genart C, Boone C, Remacle C
Laboratoire de Biologie Cellulaire, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
J Anim Sci. 1996 Nov;74(11):2745-51. doi: 10.2527/1996.74112745x.
Because tumor necrosis factor (TNF) inhibits adipose cell differentiation in vitro and affects lipid metabolism in vivo, we treated adult or newborn rats for 1 wk with daily intraperitoneal injections (100 U/g of body weight) or continuous intraperitoneal diffusion (3500 U/h) of human recombinant TNF. Three weeks after the end of treatment, the long-term effect of the cytokine was examined on adipose tissue development. Control and TNF-injected rats did not differ in growth or development of perirenal, retroperitoneal and epididymal adipose tissues. Nevertheless, the size distribution of epididymal adipocytes of adult injected rats presented a slight shift towards larger values in the cytokine group. When TNF was administered chronically, the cytokine exerted an anorectic effect, which was alleviated after the end of treatment. The weights of the excised adipose tissues were depressed (P < .025) by TNF administration. Part of this effect was due to the induced anorexia. The size distributions of the epididymal adipocytes of pair-fed and TNF-treated rats were both shifted to smaller (P < 0.01) values than for the controls. The ratio of triglycerides over total lipids was, however, reduced by TNF specifically, but only at the retroperitoneal (P < .05) and not the epididymal site. These results indicate that in contrast to acute treatment, chronic TNF treatment slightly inhibited adipose tissue development in vivo; however, most of this effect was attributable to the associated anorexia.
由于肿瘤坏死因子（TNF）在体外可抑制脂肪细胞分化，并在体内影响脂质代谢，我们对成年或新生大鼠进行了为期1周的处理，每日腹腔注射（100 U/g体重）或持续腹腔扩散（3500 U/h）人重组TNF。在处理结束3周后，检测了该细胞因子对脂肪组织发育的长期影响。对照大鼠和注射TNF的大鼠在肾周、腹膜后和附睾脂肪组织的生长或发育方面没有差异。然而，成年注射大鼠附睾脂肪细胞的大小分布在细胞因子组中呈现出向较大值的轻微偏移。当长期给予TNF时，该细胞因子发挥了厌食作用，在处理结束后这种作用得到缓解。给予TNF会使切除的脂肪组织重量降低（P <.025）。这种作用部分归因于诱导的厌食。与对照相比，配对喂养和TNF处理大鼠的附睾脂肪细胞大小分布均向较小值偏移（P < 0.01）。然而，TNF特异性地降低了甘油三酯与总脂质的比率，但仅在腹膜后部位（P <.05），而在附睾部位则没有。这些结果表明，与急性处理相反，慢性TNF处理在体内轻微抑制了脂肪组织发育；然而，这种作用大部分归因于相关的厌食。
