Buttiglione M, Revest J M, Rougon G, Faivre-Sarrailh C
Laboratoire de Génétique et de Physiologie du Développement, UMR 9943 CNRS, Parc Scientifique de Luminy, Marseille, France.
Mol Cell Neurosci. 1996;8(1):53-69. doi: 10.1006/mcne.1996.0043.
F3 is a glycane phosphatidylinositol-anchored neuronal adhesion glycoprotein which consists of immunoglobulin (Ig) domains and fibronectin type III repeats. Here we showed that total F3 or F3-Ig domains when presented as membrane components of CHO transfected cells influenced growth cone morphology, strongly inhibited outgrowth, and induced fasciculation of cerebellar granule cell axons. An F3-Ig-Fc chimera induced neurite fasciculation from cerebellar neuron aggregates when used as a coated substrate but not in the soluble form. The F3 effect on neurite elongation is highly specific for neuronal cell types since under the same experimental conditions it did not modify neurite outgrowth of hippocampal neurons and was shown to stimulate elongation of neurites from sensory neurons in both membrane-anchored and soluble form. Our results provide evidence to extend the proposed role of F3 and strongly suggest that axonal-growth-controlling molecules may quite generally exert dual actions which are likely to depend on the receptor repertoire of the responding neuron.
F3是一种糖基磷脂酰肌醇锚定的神经元粘附糖蛋白，由免疫球蛋白（Ig）结构域和纤连蛋白III型重复序列组成。我们在此表明，当作为CHO转染细胞的膜成分呈现时，总F3或F3-Ig结构域会影响生长锥形态，强烈抑制生长，并诱导小脑颗粒细胞轴突的成束。当用作包被底物时，F3-Ig-Fc嵌合体可诱导小脑神经元聚集体的神经突成束，但以可溶性形式则无此作用。F3对神经突伸长的影响对神经元细胞类型具有高度特异性，因为在相同实验条件下，它不会改变海马神经元的神经突生长，并且已表明其以膜锚定和可溶性形式刺激感觉神经元神经突的伸长。我们的结果为扩展F3的假定作用提供了证据，并有力地表明轴突生长控制分子可能普遍发挥双重作用，这可能取决于反应神经元的受体库。
