Yoo J, Stone R T, Kappes S M, Toldo S S, Fries R, Beattie C W
USDA, ARS, Roman L. Hruska U.S. Meat Animal Research Center (MARC), Clay Center, NE 68933, USA.
DNA Cell Biol. 1996 May;15(5):377-85. doi: 10.1089/dna.1996.15.377.
The cell-surface protein Fas (APO-1) is a member of the tumor necrosis factor receptor (TNFR) superfamily and transduces apoptosis following binding of Fas ligand or exposure to certain anti-Fas antibodies. We have isolated the bovine Fas (bFas) gene and determined its genomic organization and chromosomal location. Our data indicate that bFas is a single-copy gene that contains 9 exons and spans approximately 31.5 kb. The 5'-flanking region lacks conventional TATA and CCAAT elements, but contains several putative regulatory elements, including multiple copies of Sp1, AP-2, E-box, and N-box consensus sites. Linkage analysis of two (CA) dinucleotide repeat microsatellites within intron 1 and physical assignment by fluorescence in situ hybridization (FISH) placed the bFas gene on bovine chromosome 26. Collectively, these data provide a basis for understanding the regulatory mechanisms that control bFas gene expression.
细胞表面蛋白Fas（APO-1）是肿瘤坏死因子受体（TNFR）超家族的成员，在与Fas配体结合或暴露于某些抗Fas抗体后可转导细胞凋亡。我们已分离出牛Fas（bFas）基因，并确定了其基因组结构和染色体定位。我们的数据表明，bFas是一个单拷贝基因，包含9个外显子，跨度约为31.5 kb。其5'侧翼区缺乏传统的TATA和CCAAT元件，但含有几个推定的调控元件，包括多个Sp1、AP-2、E-box和N-box共有位点的拷贝。对内含子1内的两个（CA）二核苷酸重复微卫星进行连锁分析，并通过荧光原位杂交（FISH）进行物理定位，将bFas基因定位于牛26号染色体上。这些数据共同为理解控制bFas基因表达的调控机制提供了基础。
