Park H R, Park Y K
Department of Pathology, Hallym University, Seoul, Korea.
J Korean Med Sci. 1995 Dec;10(6):436-41. doi: 10.3346/jkms.1995.10.6.436.
We have performed this study to define the usefulness of an anti-human progenitor cell antigen-1(anti-CD34) to distinguish some kinds of soft tissue tumors in formalin-fixed, paraffin-embedded tissues. Sixty three cases of vascular, fibrohistiocytic, neural and other tumors were immunostained for CD34 using the streptavidin-biotin immunoperoxidase method. All of the vascular tumors including hemangiomas, epithelioid hemangioendotheliomas, hemangiopericytomas, and lymphangiomas revealed strong CD34 positivity along the cytoplasmic membranes. Among the fibrohistiocytic lesions, all of five examples of dermatofibrosarcoma protuberans showed diffuse, strong, and linear staining along the cytoplasmic processes. In contrast, none of the benign fibrous histiocytomas and malignant fibrous histiocytomas expressed CD34. CD34-positive cells with delicate dendritic processes could be identified within the normal nerves, neuromas, neurofibromas, and Antoni B areas of neurilemomas. However, all of the malignant peripheral nerve sheath tumors were uniformly negative. In addition, an epithelioid sarcoma and four cases of leiomyosarcoma revealed focal, weak positivity with anti-CD34. In conclusion, this study demonstrated variable anti-CD34 staining pattern of certain fibrohistiocytic, muscle, and neural tumors and confirmed the potential usefulness of anti-CD34 in differentiating fibrous histiocytoma from dermatofibrosarcoma protuberans. It's also helpful to diagnose epithelioid hemangioendothelioma from other epithelioid-type tumors.
我们开展这项研究是为了确定抗人祖细胞抗原-1（抗CD34）在福尔马林固定、石蜡包埋组织中鉴别某些软组织肿瘤的效用。采用链霉亲和素-生物素免疫过氧化物酶法对63例血管、纤维组织细胞、神经及其他肿瘤进行CD34免疫染色。所有血管肿瘤，包括血管瘤、上皮样血管内皮瘤、血管外皮细胞瘤和淋巴管瘤，均显示沿细胞膜呈强CD34阳性。在纤维组织细胞性病变中，所有5例隆突性皮肤纤维肉瘤均沿细胞质突起呈弥漫性、强阳性和线性染色。相比之下，良性纤维组织细胞瘤和恶性纤维组织细胞瘤均未表达CD34。在正常神经、神经瘤、神经纤维瘤和神经鞘瘤的Antoni B区可识别出具有纤细树突状突起的CD34阳性细胞。然而，所有恶性外周神经鞘瘤均呈一致阴性。此外，1例上皮样肉瘤和4例平滑肌肉瘤显示抗CD34呈局灶性弱阳性。总之，本研究显示某些纤维组织细胞性、肌肉性和神经性肿瘤具有不同的抗CD34染色模式，并证实抗CD34在鉴别纤维组织细胞瘤和隆突性皮肤纤维肉瘤方面具有潜在效用。它对从上皮样型肿瘤中诊断上皮样血管内皮瘤也有帮助。
