Morozov I I
Biofizika. 1996 May-Jun;41(3):636-41.
The influence of aggregate condition, nutrient composition and tonicity of incubation medium on recovery from damages induced by heat at 52 degrees C Escherichia coli B/r and Escherichia coli BS-1 cells was studied. The cells were shown to be recovered from heat damages only in liquid and isotonic medium. The observed increase in a number of viable organisms was not greatly depended on source of energy, plastic material and macroelements of incubation medium. The recovered cells showed increase of osmotic tolerance to hyper- and hypotonic shock similar to intact cells. No increase in survivors was observed when heated cells were incubated on solid and/or anisotonic medium. The additional cell killing and high osmosensitivity may be seen in this case. The role of the system of osmotic homeostasis, including membrane state, in the modification of cell viability and osmoresistance of heated bacterial cells was discussed on the base of the data obtained.
研究了聚集状态、营养成分和孵育培养基的张力对52℃加热诱导损伤的大肠杆菌B/r和大肠杆菌BS-1细胞恢复的影响。结果表明，细胞仅在液体等渗培养基中才能从热损伤中恢复。观察到的活生物体数量增加在很大程度上不依赖于能量来源、塑料材料和孵育培养基的大量元素。恢复的细胞对高渗和低渗休克的渗透耐受性增加，类似于完整细胞。当加热的细胞在固体和/或非等渗培养基上孵育时，未观察到存活者增加。在这种情况下可能会出现额外的细胞杀伤和高渗透压敏感性。基于所获得的数据，讨论了包括膜状态在内的渗透稳态系统在改变加热细菌细胞的活力和渗透压抗性中的作用。
